(ABC4) – Since the early 1980s, Forbes has published an annual list of the wealthiest 400 American citizens. Around September for the last 39 years, Forbes has released the list. For eight years, and then the last 25 years, former President Donald Trump has been on that list.

Not this year.

Forbes’ Dan Alexander explains Trump, worth an estimated $2.5 billion, fell $400 million short of the cutoff to make this year’s list. In 2020, Trump landed in the No. 339 spot. According to Forbes, since the start of the COVID-19 pandemic, Trump is down $600 million.

The last time Trump did not make the list was in 1995. As Forbes reports, Trump was on the first list in 1982, then fell off the list from 1990 to 1995 before rejoining in 1996.

Trump’s wealth has often been a topic of conversation. In a 2018 story, the New York Times challenged Trump’s claims of self-made wealth by documenting how his father, Fred, had given him at least $413 million over the decades, including through tax avoidance schemes. Mary Trump, the former president’s niece, identified herself in a book published last year as the source of the documents provided to the Times.

SLIDESHOW: The top five on the Forbes 400 for 2021 from first to fifth

  • Jeff Bezos
    FILE – In this Thursday, May 9, 2019, file photo, Jeff Bezos speaks at an event before unveiling Blue Origin’s Blue Moon lunar lander, in Washington. On Monday, July 12, 2021, the Federal Aviation Administration approved Blue Origin’s attempt to launch people into space. (AP Photo/Patrick Semansky, File)
  • FILE – In this Dec. 1, 2020, file photo, SpaceX owner and Tesla CEO Elon Musk arrives on the red carpet for the Axel Springer media award, in Berlin. (Hannibal Hanschke/Pool Photo via AP, File)
  • FILE – This Oct. 25, 2019 file photo shows Facebook CEO Mark Zuckerberg speaking at the Paley Center in New York. Zuckerberg donated $400 million to help fund election offices as they scrambled to deal with the coronavirus pandemic late last summer. At least eight GOP-controlled states have passed bans on donations to election offices this year as Republicans try to block outside funding of voting operations. (AP Photo/Mark Lennihan, File)
  • FILE – In this April 21, 2018, file photo, Bill Gates speaks in Washington. Microsoft co-founder Bill Gates said Friday, March 13, 2020 he is stepping down from the company’s board to focus on philanthropy. Gates was Microsoft’s CEO until 2000 and since then has gradually scaled back his involvement in the company he started with Paul Allen in 1975. (AP Photo/Jose Luis Magana, File)
  • FILE – In this Nov. 2, 2015, file photo, Google co-founder Larry Page speaks at the Fortune Global Forum in San Francisco. Page has gained New Zealand residency, officials confirmed Friday, Aug. 6, 2021, stoking debate over whether extremely wealthy people can essentially buy access to the South Pacific country. (AP Photo/Jeff Chiu, File)

In a statement to NBC News, Mary Trump said of her uncle, “I think he is a loser, and he is going to throw anything against the wall he can. It’s desperation. The walls are closing in and he is throwing anything against the wall that he thinks will stick. As is always the case with Donald, he’ll try and change the subject.”

Trump, the only president in modern times not to make his tax filings public, paid no federal income taxes in 10 of the past 15 years. As the Associated Press reports, he campaigned for office as a billionaire real estate mogul and successful businessman. Trump paid just $750 in federal income taxes the year he ran for president and in his first year in the White House, according to a 2020 report in The New York Times.

Forbes reports Trump’s net worth in 2021 – $2.5 billion – in the same as it was in 2020 and hasn’t been this low since 2009. That year, Trump’s net worth was estimated to be at $2 billion. Alexander says Trump can blame himself for the dip in his net worth – and falling off the Forbes 400. Forbes shows Trump’s ranking on the list began falling after he won the presidency.

Now, for the first time in 25 years, Trump is off the list. To see the full Forbes 400, click here.

The Associated Press contributed to this report.